【文献标题】Effects of dietary supplementation of inulin on rumen fermentation and bacterial microbiota, inflammatory response and growth performance in finishing beef steers fed high or low-concentrate diet

【作者】Ke Tian,Junhui Liu,Yawang Sun,et.al

【作者单位】西南大学（Southwest University）

【文献中引用产品】

牛脂多糖结合蛋白(LBP)ELISA试剂盒

牛结合珠蛋白/触珠蛋白(Hpt/HP)ELISA试剂盒

牛血清淀粉样蛋白A(SAA)ELISA试剂盒

牛C反应蛋白(CRP)ELISA试剂盒

【关键词】Inulin,Rumen,Bacterial microbiota,Lipopolysaccharide,Beef steer

【DOI】doi.org/10.1016/j.anifeedsci.2019.114299

【影响因子(IF)】5.0

【出版期刊】《Animal Feed Science and Technology》

【产品原文引用】

Plasma cytokines and APPs assay

The bovine-exclusive ELISA kit was used to measure the concentrations of plasma IL-1β, IL-6, IL-8 and TNF-α. The bovine ELISA kits were purchased from Shanghai Sinobest Biotechnology Co., Ltd. (Shanghai, China). APPs including LPS-binding protein (LBP),haptoglobin (Hp), serum amyloid A (SAA) and C-reactive protein (CRP) were determined using the bovine ELISA kits purchased from Shanghai Jinma Biotechnology Co., Ltd. (Shanghai, China). The determination was conducted according to the manufacturer’s instructions. All samples including the standards were tested in duplicate, and the optical density values were read on a microplate reader (Bio-Rad, xMarkTM, USA) at 450 nm.

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