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技术文献:小鼠IL-1β,IL-6,等ELISA试剂盒技术文献
时间:2021/3/17      阅读:962

【文献标题】Rational Avoidance of Protease Cleavage Sites and Symmetrical End-Tagging Significantly Enhances the Stability and Therapeutic Potential of Antimicrobial Peptides
【作者】Yongjie Zhu, Changxuan Shao, Guoyu Li,et.al
【作者单位】东北农业大学( Northeast Agricultural University)
【文献中引用产品】
小鼠白介素1β (IL-1β)ELISA试剂盒
小鼠白介素6(IL-6)ELISA试剂盒
小鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒
【DOI】dx.doi.org/10.1021/acs.jmedchem.0c00583
【影响因子(IF)】2.57
【出版期刊】《MED CHEM》
【产品原文引用】
In Vivo Efficacy Assay (Peritonitis-Sepsis Mode
Twenty-four C57BL/6 female mice (Liaoning Changsheng Biotechnology Co., Ltd.,Benxi, China) aged 6?7 weeks and weighing 20?22 g were randomly divided into the following three groups (n = 8): (a) control group(saline), (b) infection group (E. coli), and (c) treatment group (E. coli + II-I4-II). After 3 days of prefeeding, 100 μL of saline was injected into the peritoneal cavity of mice in the control group, while other mice received 100 μL of E. coli 25922 (~1 × 108 CFU/mL) to initiate infection. The treatment group was treated with II-I4-II (10 mg/kg) via intraperitoneal injection 1 h after infection, while the control group and infection group received saline. Then, 12 h after the infection, the mice were euthanized. At the same time, the liver, spleen, kidney, and lung of mice were taken out and weighed to analyze the bacterial load in those organs. For histopathological examination, liver, spleen, kidney, and lung sections were fixed in  4% paraformaldehyde, embedded in paraffin, and stained with hematoxylin and eosin. The level of IL-1β, IL-6, and TNF-α in the serum was analyzed with ELISA kits (Shanghai Jinma)


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