【文献标题】Crocetin Downregulates the Proinflammatory Cytokines in Methylcholanthrene-Induced Rodent Tumor Model and Inhibits COX-2 Expression in Cervical Cancer Cells

【作者】Bing Chen, Zhao-Hui Hou, Zhe Dong,et.al

【作者单位】Air Force General Hospital（空军总医院）

【文献中引用产品】

小鼠白介素1β (IL-1β)ELISA试剂盒

小鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒

【关键词】

【DOI】doi.org/10.1155/2015/829513

【影响因子(IF)】3.24

【出版期刊】《Biomed Research International》

【产品原文引用】

 Measurement of IL-1β and TNF-α Level. The concentration of IL-1β and TNF-α was determined using a commercial ELISA kit (Shanghai Jinma Biological Technology, Inc.,China) following the manufacturer’s instruction.

Assessment of iNOS Protein Histochemistry. The tumors were dissected free from soft tissues at the end of the study  and collected on chrome gel subbed slides. Tissue sections were fixed in 50% acetone in phosphate-buffered saline for  3 minutes at room temperature and endogenous peroxidase activity was blocked by 1.5% H2O2 in methanol for 15  minutes. The iNOS protein was determined using a commercial ELISA kit (Shanghai Jinma Biological Technology, Inc.,China) following the manufacturer’s instruction.

Crocetin Inhibited COX-2 Production in HeLa Cells. Analysis of COX-2 production was performed using an ELISA kit according to manufacturer’s instructions (Shanghai Jinma Biological Technology, Inc., China). HeLa cells were treated with various concentrations of crocetin (1, 10, 50, and100 mol/L) for 24 h. Cell culture supernatants were collected to measure the concentration of COX-2. Production of COX-2 was normalized to protein concentrations.

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