【文献标题】Abnormal Bone Metabolism May Be a Primary Causative Factor of Keel Bone Fractures in Laying Hens

【作者】Haidong Wei , Yanqing Chen , Haoyang Nianet.al

【作者单位】Northeast Agricultural University（东北农业大学）

【文献中引用产品】

鸡25羟维生素-D3（25(OH)D3）ELISA试剂盒

鸡1，25-二羟基维生素-D3(1,25-(OH)2D3)ELISA试剂盒

鸡甲状旁腺激素（PTH）ELISA试剂盒

鸡降钙素(CT)ELISA试剂盒

鸡骨钙素(OCN)ELISA试剂盒

鸡碱性磷酸酶(ALP)ELISA试剂盒   活性

鸡抗酒石酸酸性磷酸酶 (TRAP)ELISA试剂盒   活性

鸡骨保护素（OPG）ELISA试剂盒

【关键词】laying hens; keel bone damage; bone metabolism; bone health; furnished cage

【DOI】doi.org/10.3390/ani11113133

【影响因子(IF)】2.99

【出版期刊】《Animals 》

【产品原文引用】

2.6. Determination of Serum Ca and P Metabolism-Related Markers .The concentration of serum Ca of the laying hens was measured using a microplate reader of the Ca assay kit (Kit number: C004-2-1), according to the manufacturer’s instruction (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Conversely, serum Pconcentration was determined by the molybdenum blue method using a serum P assay kit (Kit number: C006-1-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China).Consistently, the concentrations of serum 25-OHD3 (Kit number: JB278-Ch) and 1,25-(OH)2D3 (Kit number: JB331-Ch) (Shanghai Jinma Laboratory Equipment Corporation Ltd, Shanghai, China), parathyroid hormone (PTH) (Kit number: ML002803, Shanghai enzyme-linked Biotechnology Corporation Ltd, Shanghai, China), and calcitonin (CT) (Kit number: JB135-Ch, Shanghai Jinma Laboratory Equipment Corporation Ltd, Shanghai, China) were determined using the enzyme-linked immunosorbent assay (ELISA) method following the manufacturer’s instructions for the corresponding kits.

2.7. Determination of Serum Osteoblast and Osteoclast-Related Markers

The levels of bone formation (osteoblast activity) markers osteocalcin (OC) (Kit number: JB162-Ch) and ALP (Kit number: JB329X-Ch), and bone absorption (osteoclast activity) markers TRAP (Kit number: JB330X-Ch) and osteoprotegerin (OPG) (Kit number: JB163-Ch) in the serum of the laying hens were measured by ELISA method using a microplate reader of the corresponding ELISA kits (Shanghai Jinma Laboratory Equipment Corporation Ltd, Shanghai, China). The activity of serum corticosterone (CORT) was detected with an ELISA kit (Kit number: H205-1-2) following the manufacturer’s instruction (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). A total of 50 microliters (μL) of serum sample (including 10 μL of serum and 40 μL of sample dilution) from each experimental sample were added into the micropore for physiological indicators evaluation. Moreover,a standard curve was obtained using the standard solutions of different concentrations for each marker to quantify the concentrations of the experimental samples. The optical density values were then measured at a wavelength of 450 nm using a microplate reader (Biotek Instrument Inc., Winooski, VT, USA).

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