【文献标题】Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus

【作者】Xiaofeng Ren、Pengchong Liet.al

【作者单位】Northeast Agricultural University（东北农业大学）

【文献中引用产品】

猪流行性腹泻病毒（PEDV）ELISA试剂盒

【关键词】Porcine epidemic diarrhea virus RT-LAMP RT-PCR

【DOI】10.1007/s11262-011-0570-3

【影响因子(IF)】1.6

【出版期刊】《Virus Genes 》

【产品原文引用】

 Moreover, the sensitivity between the RT-LAMP and conventional ELISA was compared using the inactivated PEDV as template. The former is more sensitive than the latter. Two reports have pointed out that the detection limit of RT-PCR for PEDV was 102.0 TCID50/ml [29, 30]. Detection limit of a commercially available ELISA kit (Jinma, Shanghai) used in China was 0.1 ng/ml, which was the same as the detection limit of the RT-LAMP developed in this study. This result further confirmed the sensitivity of the RT-LAMP for amplifying the N gene of PEDV. Nonetheless, when the authors used these methods to detect PEDV from clinical samples, the sensitivity of RT-LAMP was somewhat higher than RT-PCR and had a similar sensitivity with ELISA. More experiments are needed to clarify this point in the future; however, the RT-LAMP still has advantages including simplicity, rapidity, and convenience.

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