【文献标题】Anti-inflammatory effects of Neurotoxin-Nna, a peptide separated from the venom of Naja naja atra

【作者】Yeping Ruan、Li Yao、Bingbing Zhanget.al

【作者单位】 Zhejiang Chinese Medical University（浙江中医药大学）

【文献中引用产品】

大鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒

大鼠白介素1β (IL-1β)ELISA试剂盒

【关键词】Neurotoxin-Nna, Anti-inflammatory, Antinociceptive, Tumor necrosis factor alpha, Interleukin 1 beta, Nuclear factor kappa B

【DOI】10.1186/1472-6882-13-86

【影响因子(IF)】4.78

【出版期刊】《BMC Complementary and Alternative Medicine 》

【产品原文引用】

Measurement of IL-1β, TNF-α level and activation of NF-κB。The serum concentration of tumor necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β) were measured  using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Shanghai Jinma Biological Technology, Inc., China) following the manufacture’s instruction.Activated Nuclear factor kappa B (NF-κB) was tested via ELISA-technique using the PathScan Phospho-NFκB p65 (Ser536) Sandwich ELISA Antibody Pair (Shanghai Yubo Biological Technology, Inc., China), following the manufacture’s instruction. Briefly, the activated NF-κB specifically binds to oligonucleotides corresponding to NF-κB consensus binding sites and immobilized in a 96-well plate. Bound NF-κB is detected with an anti-p65 antibody. Addition of a secondary HRP-conjugated antibody provides sensitive colorimetric readout quantified by spectrophotometry at 450 nm.

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