【文献标题】Vitamin E-Inhibited Phoxim-Induced Renal Oxidative Stress and Mitochondrial Apoptosis In Vivo and In Vitro of Piglets

【作者】Jing Zhang, Yuecheng Sun, Wentao Songet.al

【作者单位】Northeast Agricultural University（东北农业大学）

【文献中引用产品】

猪丙二醛（MDA）ELISA试剂盒

猪总抗氧化能力（T-AOC）检测试剂盒

猪超氧化物歧化酶(SOD)ELISA试剂盒

猪过氧化氢酶（CAT）ELISA试剂盒

猪谷胱-甘肽过氧化物酶（GSH-Px）ELISA试剂盒

猪细胞-色素C氧化酶ELISA试剂盒

【关键词】phoxim; vitamin E; oxidative stress; mitochondrion; apoptosis

【DOI】doi.org/10.3390/antiox12112000

【影响因子(IF)】5.9

【出版期刊】《antioxidants》

【产品原文引用】

Oxidation–Antioxidant Parameters

In vivo, the serum ROS levels were determined in the piglets by an enzyme-linkedimmunosorbent assay (ELISA), which was operated strictly according to the instructions of the kit (Shanghai Jinma Co., Ltd., Shanghai, China) on a Labsystems Multiskan MS (Finland). The antioxidant enzyme parameters were tested using an ultraviolet spectrophotometer (UV-2410PC model, Shimadzu Corp., Kyoto, Japan) according to the instructions of the commercial diagnostics kit (Nanjing Jiancheng Biotechnology Co., Ltd., Nanjing, China).

The parameters that were evaluated in the kidney were malondialdehyde (MDA), total antioxidant capacity (T-AOC),superoxide dismutase (SOD), glutathione (GSH), catalase(CAT), and glutathione peroxidase (GSH-Px).

Mitochondrial Function

For mitochondrial function, the activities of succinate dehydrogenase (SDH), cytochrome c oxidase (COX), and Ca2+-Mg2+-ATPase in the PK15 cells were determined by ELISA. The enzyme activity was detected by a Labsystems Multiskan MS (Finland) system in strict accordance with the instructions of the kit (Shanghai Jinma Co., Ltd.,Shanghai, China).

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