【文献标题】Anti-Inflammatory Activity of Water-Soluble Polysaccharide of Agaricus blazei Murill on Ovariectomized Osteopenic Rats

【作者】Peng Wang, Xiao-Tao Li, Lei Sun et.al

【作者单位】 Harbin Medical University（哈尔滨医科大学）

【文献中引用产品】

大鼠白介素1β (IL-1β)ELISA试剂盒

大鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒

【关键词】无

【DOI】dx.doi.org/10.1155/2013/164817

【影响因子(IF)】2.6

【出版期刊】《Evidence-Based Complementary and Alternative Medicine》

【产品原文引用】

Estimation of Serum IL-1β and TNF-α Level. Serum samples were disintegrated in 5 volumes of ice-cold RIPA buffer. After incubation on ice for 30 minutes, samples were centrifuged twice at 20,000 Xg for 15 minutes at 4°C. The resulting supernatants were used for assay. The concentration of IL-1β and TNF-α was determined using a commercial ELISA kit (Shanghai Jinma Biological Technology, Inc., China) following the manufacture’s instruction.

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