【文献标题】Agaricoglycerides Protect against Hepatic Ischemia/Reperfusion Injury by Attenuating Inflammatory Response, Oxidative Stress,and Expression of NF-??B

【作者】Xiang-qian Zhao, Bin Liang, Yang Liu et.al

【作者单位】Chinese PLA General Hospital（中国人民-解放-军总医院）

【文献中引用产品】

小鼠白介素1β (IL-1β)ELISA试剂盒

小鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒

【关键词】无

【DOI】doi.org/10.1155/2015/142736

【影响因子(IF)】2.6

【出版期刊】《Evidence-Based Complementary and Alternative Medicine》

【产品原文引用】

Estimation of Liver TNF-α and IL-1β. Liver samples were disintegrated in 5 volumes of ice-cold RIPA buffer. After incubation on ice for 30 minutes, samples were centrifuged twice at 20,000 ×g for 15 minutes at 4°C. The resulting supernatants were used for assay. The hepatic concentration of TNF-α and IL-1β was measured by the way introduced by Moon et al. [12] using a commercial ELISA kit (Shanghai Jinma Biological Technology Inc., China) following the manufacture’s instruction.

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