【文献标题】Effect of serum from postmenopausal women with osteoporosis exhibiting the Kidney—Yang deficiency pattern on bone formation in an hFOB 1.19 human osteoblastic cell line.

【作者】YACHAN LI1, WENNA LIANG1, XIHAI LI et.al

【作者单位】Fujian University of Traditional Chinese Medicine（福建中医药大学）

【文献中引用产品】

人雌二醇(E2)ELISA试剂盒

人胰岛素样生长因子结合蛋白1(IGFBP-1)ELISA试剂盒

【关键词】无

【DOI】10.3892/etm.2015.2616

【影响因子(IF)】2.4

【出版期刊】《Experimental and Therapeutic Medicine 》

【产品原文引用】

The serum concentration of estradiol (E2), OPG, and insulin?like growth factor 1 (IGF1) was assessed using ELISA (Shanghai Jinma Biological Technology Co., Ltd, Shanghai, China). All commercial assays were performed according to the manufacturer's instructions.Briefly, ELISA plates were percolated with mouse anti-human immunoglobulin G,and standards, and samples were loaded into the wells and incubated for 1 h at room temperature.HRP—conjugated anti-human E2, OPG and IGF-1 detection antibodies were added and incubated at room temperature for 1 h. The reaction was visualized through color development and the absorbance (optical density) was measured at a 450?nm wavelength on an ELISA reader (Model EXL800; BioTek Instruments, Inc.). The conversion of optical density units for the study samples to concentration was achieved through a computer software-mediated comparison with a standard curve using the KC Junior (BioTek Instruments, Inc.)

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