日本MBL公司人嗜酸细胞阳离子蛋白（ECP）试剂盒说明书
 

For Research Use Only. Not for use in diagnostic procedures.

2. Summary and Explanation Eosinophil Cationic protein （ECP）, Eosinophil derived Neurotoxin （EDN） and Major Basic Protein （MBP） are known to be major protein-mediators derived from activated eosinophils. ECP and EDN are found in matrix of granules in eosinophils whereas MBP is found in core of granules. ECP and EDN are members of the ribonuclease A superfamily. ECP and MBP have high cytotoxicity. These three proteins are highly cationic proteins with PI 10.8-10.9. Activated eosinophile play an important role in the late asthmatic response and in the asthmatic airway inflammation. As ECP is secreted from activated eosinophils, ECP can be a marker of eosinophile activation and degranulation. “MESACUP ECP TEST” is the reagent for measuring specifically with high sensitivity ECP by ELISA.

3. Principle

MBL MESACUP ECP TEST measures human ECP by sandwich ELISA. This ELISA detects human ECP with a minimum detection limit of 0.125 ng/ml and does not cross-react with EDN. In the wells coated with anti-human ECP monoclonal antibody, samples to be measured or standards are incubated. After washing, a peroxidase conjugated anti-human ECP polyclonal antibody is added into the microwells and incubated. After another washing, the peroxidase substrate is mixed with the chromogen and allowed to incubate for an additional period of time. An acid solution is then added to each well to terminate the enzyme reaction and to stabilize the developed color. The optical density （O.D.） of each well is then measured at 450 nm using a microplate reader. The concentration of ECP is calibrated from a standard curve based on reference standards.