Description
Analysis Note Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
Biochem/physiol Actions PHA consists of two molecular species, an erythroagglutinin （PHA-E） which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin （PHA-L） which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.1,2
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.4 PHA-M is the mucoprotein form.4 Conjugates are prepared from the corresponding purified lectins.
Quality Mitogenic activity at <10 μg per mL.

Properties
product line BioXtra
form lyophilized powder
potency <16 μg per mL agglutination activity
purified by affinity chromatography
total impurities salt, essentially free
storage temp. 2-8°C

Safety
Safety Statements 22-24/25
RTECS TJ3470000