Description
Application 3,3′,5,5′-Tetramethylbenzidine （TMB） is a noncarcinogenic substitute （Ames test negative） for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 （resulting in a yellow color）, and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8
Other Notes Standard for determining aromatic amines released from azo-dyes in commodities9

Properties
grade standard for GC
  puriss. p.a.
assay ≥99.0% （GC）
shelf life  （limited shelf life, expiry date on the label）
mp 166-171 °C
  168-171 °C（lit.）

Safety
Personal Protective Equipment dust mask type N95 （US）, Eyeshields, Faceshields, Gloves
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36
WGK Germany 3
RTECS DV2300000